Some specific uses of deep-well plate
The
deep-well plate is a multi-well plate whose well depth is greater than the good width. These plates are commonly used in laboratories for various applications such as sample preparation, cell culture, and high-throughput screening.
1. Sample preparation: Deep well plates can be used for sample preparation, such as mixing, stirring, and incubation of samples. Deep wells provide ample volume for samples to be processed, and the plate can be easily agitated or mixed using a shaker or vortex mixer.
2. Cell culture: Deep well plates are usually used for cell culture experiments, especially for high-throughput cell detection. The wells in the plate can be coated with various substances to promote cell attachment and growth, and the wells provide enough volume for the cells to grow and divide over time.
3. Protein expression and purification: Deep well plates can be used for small-scale protein expression and purification experiments. Wells can be filled with bacterial or mammalian cell cultures expressing specific proteins, and proteins can be purified using various chromatographic techniques.
4. DNA/RNA extraction and purification: deep well plates can also be used for DNA or RNA extraction and purification. Deep wells allow efficient lysis of cells or tissues, and samples can be processed using various purification methods, such as column-based or bead-based methods.
How many samples can you run on 8-strip tubes?
8-strip tubes are tubes that consist of eight individual tubes joined together. Each individual tube holds a volume of approximately 0.2 to 0.3 mL, depending on the type of tube.
Typically, each of the eight separate tubes in an 8-tube strip can be used as a separate sample, and you can run up to eight samples at a time on a single 8-tube strip.
8-strip tubes are commonly used in molecular biology applications such as polymerase chain reaction (PCR) and other DNA amplification techniques. In PCR, each tube in an 8-tube strip can hold a reaction mixture consisting of a DNA template, primers, and other reagents. Eight reactions in eight tubes can then be scaled up and analyzed simultaneously using a thermal cycler.
Are all V-bottom & 8-strip tubes the same size?
No, not all
V-bottom & 8-strip tubes are the same size. Tube size and shape can vary depending on the manufacturer and specific application.
V-bottom tubes are named for their tapered bottom that resembles the letter "V". These tubes come in a variety of sizes and capacities, from as small as 0.2ml to as large as 50ml or larger. The diameter of the tubes can also vary, with some tubes being narrower and taller, while others are wider and shorter. The size of the test tube depends on the volume of sample used and the type of equipment expected to be used.
Likewise, the 8 tubes come in different sizes, although they are usually standardized to fit most thermal cyclers. Tubes may vary in diameter and height, but they are usually designed to hold a volume of 0.2 to 0.3 ml per tube.
Selecting the proper size and shape of the tube for a specific application is important to ensure accurate and reproducible results. It is also important to ensure that the tubes are compatible with the equipment being used, such as thermal cyclers, centrifuges, or other laboratory instruments.
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