Precautions For The Use Of Freezing Tube

Freezing Tube is also known as a strain storage tube, magnetic bead storage tube, and magnetic bead cryopreservation tube. The commonly used strain preservation methods in the microbiology laboratory include the milk method, the glycerol method, and the slant method. The complexity varies greatly, and the effect varies greatly. At present, most laboratories in China make their own strain preservation tubes, which not only increases the work intensity but also due to various conditions, the strain preservation effect is not always satisfactory. Therefore, it is necessary to master the use of cryopreservation tubes and some precautions, so as to play a great role. Precautions for the use of Freezing Tube:

1. The storage environment of cryopreservation tubes. Unused cryopreservation tubes can be stored at room temperature or 2-8°C for 12 months; inoculated cryotubes can be stored at -20°C, and good strains can be stored within 12 months. Effect: The inoculated cryopreservation tube can be stored at -80°C, and the strain can be preserved within 24 months.

2. Storage time of cryovials, unused cryovials can be stored at room temperature or 2-8°C; inoculated cryovials can be stored at -20°C or -80°C.

3. The steps of using the cryopreservation tube, pick the fresh culture from the pure bacterial culture and make it into a bacterial suspension with a McFarland turbidity of about 3-4 and inoculate it into the bacterial strain storage tube; tighten the storage tube and turn it upside down. 4-5 times to emulsify the bacteria, do not shake; put the preservation tube in the refrigerator (-20℃--70℃)