Pipette Tips Manufacturers Introduces The Correct Use Of Pipettes

The pipette is actually a measuring instrument, mainly used to measure the volume of the solution. It is convenient and simple to use, and the measurement effect is relatively accurate. However, attention should also be paid to the use of the liquid pipe. Only the correct method can achieve The effect better, so the following Pipette Tips Manufacturers introduce how to use the pipette correctly and the problems that need attention.

1. Steps to use
According to the volume and requirements of the solution to be pipetted, select a pipette of appropriate specifications for use. Pipettes are generally used to accurately pipette solutions in titration analysis, and pipettes are generally used when the reaction needs to control the amount of test solution added.

2. Check the instrument
Check whether the nozzle and tip of the pipette are damaged, if damaged, it cannot be used;

3. Clean the instrument
After rinsing with tap water, soak in the chromic acid washing solution. The operation method is as follows: hold the upper end of the pipette or pipette at a suitable position with the right hand, the index finger is close to the upper opening of the pipette, and the middle finger and ring finger are spread to hold the outside of the pipette. Hold the thumb on the inside of the pipette between the middle finger and the ring finger, and the little finger relaxes naturally; hold the ear cleaning ball in the left hand, hold the ear cleaning ball in the fist position, hold the ear suction ball in the palm, with the tip pointing down, hold the ear suction ball tightly, and discharge the ball Air, insert the tip of the suction ear ball into or close to the top of the pipette (pipette), and be careful not to leak air. Slowly release the fingers of the left hand, slowly suck the washing liquid into the tube until it reaches the part above the scale line, remove the suction ear ball, and quickly block the upper opening of the pipette (pipette) with the index finger of the right hand, wait for a while, and then put the washing liquid into the tube. Return the liquid to the original bottle. And rinse the inner and outer walls of the pipette (pipette) with tap water until no water drops hang, then wash with distilled water 3 times, and drain the water for later use.

4. Draw up the solution
Shake the solution to be imbibed, pour a small part of the solution to be imbibed into a clean and dry small beaker, use filter paper to absorb the moisture inside and outside the cleaned pipette tip, and insert it into the small beaker to absorb the solution, when When the pipette is sucked to 1/3 of its capacity, press the mouth of the pipette with your right index finger immediately, take it out, hold it horizontally and turn the pipette to make the solution flow all over the inner wall of the pipette, and discharge the solution from the tip of the lower end into the waste liquid inside the cup. In this way, the solution can be absorbed after rinsing 3-4 times.

5. Adjust the liquid level
Take another clean small beaker with your left hand, put the tip of the pipette against the inner wall of the small beaker, keep the small beaker tilted, keep the pipette vertical, and keep the scale line and line of sight horizontal (the left hand cannot touch the pipette). Slightly loosen the index finger (you can turn the pipette or pipette slightly), so that the solution in the tube flows out slowly from the lower mouth. When the liquid level reaches the scale line, press the right index finger firmly, pause for a moment, and then press the above method to dispense the solution. Put the bottom line of the meniscus until it is tangent to the upper edge of the marking line, and immediately press the nozzle tightly with your index finger. Put the tip close to the inner wall of the beaker, move a little towards the mouth of the beaker, and remove the droplet at the tip. Carefully transfer the pipette or pipette to the container receiving the solution.